Video Optimizer Tutorial: Actin Motility
Data set and source
This is data collected by Sreeja Asokan. It is Actin surfing on a surface with a flow of 15 microliters per minute.
One frame from the unoptimized video is shown here:
Suggested procedure for optimizing
Check the show_gain_control box to bring up the gain control panel. On that control panel, adjust the clip_low to map 0.72 to black and clip_high to map 0.75 to white. This reduces the noise and increases the contrast. The optimized image corresponding to the above image is shown here:
The resulting optimized flow can also be usefully displayed using the ScalarStack program with the “Sreeja” technique.