Fluorescence Microscope Simulation of the Mitotic Spindle

February 1, 2009
The mitotic spindle model (Yeh et al., 2008). (a) Side-on view; (b) end-on view. Helices represent the chromatin labeled with green fluorescing protein. Experimental and noise-free simulated images in side-on orientation (c-d) and end-on orientation (e-f). Differences in background between experimental and simulated images are caused by external chromatin not accounted for in the model. Structural variation in real specimens account for shape discrepancies.

Mitotic Spindle

January 22, 2009
Model of the mitotic spindle and an isosurface from a 3D stack of fluorescence images of a real mitotic spindle. The model has been manually placed relative to the image data. A "make it better" button in the FluoroSim module of the CISMM Microscope Simulator will optimize the fit of the geometric model to the image information by forward simulation of the fluorescence microscope imaging process.
Model of the mitotic spindle and an isosurface from a 3D stack of fluorescence images of a real mitotic spindle. The model has been manually placed relative to the image data.