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This tutorial shows you how to load time-varying datasets into ImageSurfer2.

To make ImageSurfer think an input is a time-varying data, you just have to name your data at different time steps sequentially. For example, suppose you have ten file, each of which is from a common dataset at a different time step. They should be named

stack001.tif
stack002.tif

stack010.tif

You can use ImageJ forĀ conversionĀ if your dataset uses a different naming scheme.

In the file loading dialog, ImageSurfer will then aggregate these files together (since they have a common prefix) as a single entry. Load the aggregation entry will load the whole time steps. When you are prompt with a ‘File loader’ option, you will see two ‘TIFF Image Files’ entries. Choose the first one.

After opening the data, rendering it in some visualization representation, e.g. volume rendering, an then you can play the animation by clicking the ‘Play’ button in the tool bar.

Screenshot-from-2014-06-30-121310.