ImageSurfer 2: Load Dentritic Spine Data Set
Download imagesurfer dendritic_spines.zip
This data is from Alain Burette at Richard Weinberg’s laboratory at the University of North Carolina at Chapel Hill. The DiO data shows the stained membrane of a dentrite, with spines coming out of it. The PMCA2 data set shows where calcium channels are.
Unzip the file to a directory of your choosing. To load a data set, click File -> Open
, and navigate to the directory containing the unzipped data. There are groups of images: DiO and PMCA2. To load the DiO data, double click on dio...tif
. Double click on “Collection of slides” from the pop-up dialog (It’s “TIFF Volume File Format” in older versions of ImageSurfer 2). Click the Apply
button in the Properties panel on the left. A white wire frame should show up, representing the bounding box of the DiO data.
To load the PMCA data set, click File -> Open again, double click on pmca2...tif
, double click on “TIFF 2D Slice Sequence” (if in an older version, it’s TIFF Volume File Format)
, and finally, hit Apply
. Pmca is now also showing in the Pipeline Browser.