A 2.8-micron diameter bead attached to slowly-beating cilia is tracked by the laser tracking and position feedback systems of the 3DFM. For the first two cycles, the bead is not tracked; it and the other two beads near the bottom of the image move as the cilia beat. As the tracking feedback kicks in, the background moves while the bead stays fixed in the center of the “+” sign. Note that the point on the bead that is tracked is slightly out of the focal plane of the optical microscope, so the three beads get slightly fuzzy while tracking is operating.

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